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ATCC
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ATCC
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MARINPHARM gmbh
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Image Search Results
Journal: Pharmaceuticals
Article Title: Isoliensinine Induces Ferroptosis in Urothelial Carcinoma Cells via the PI3K/AKT/HIF-1α Axis: Molecular Evidence from Next-Generation Sequencing
doi: 10.3390/ph18071008
Figure Lengend Snippet: Evaluation of the effects of isoliensinine on the viability, proliferation, and apoptosis pathways of urothelial carcinoma (UC) cells. An MTT assay was performed to measure the viability of T24 ( A ) and UMUC3 ( B ) cells after treatment with isoliensinine (0, 20, 40, and 80 μM) for 48 h. A colony formation assay was performed on T24 ( C ) and UMUC3 ( D ) cells after isoliensinine treatment (0, 20, and 40 μM) for 2 days, followed by incubation in untreated medium for 7 days. ( E ) Flow cytometry using annexin V/propidium iodide dual staining showed that apoptosis was induced in UC cells by isoliensinine treatment at 0, 20, and 80 μM for 48 h. ( F ) Transformation of the cell distribution in E to a bar chart, shown as mean ± SD% (* p < 0.05, *** p < 0.001).
Article Snippet: In this study,
Techniques: MTT Assay, Colony Assay, Incubation, Flow Cytometry, Staining, Transformation Assay
Journal: Pharmaceuticals
Article Title: Isoliensinine Induces Ferroptosis in Urothelial Carcinoma Cells via the PI3K/AKT/HIF-1α Axis: Molecular Evidence from Next-Generation Sequencing
doi: 10.3390/ph18071008
Figure Lengend Snippet: RNA sequencing analysis of isoliensinine-treated urothelial carcinoma (UC) cells. ( A , B ) Volcano plots for differentially expressed genes (DEGs) detected in isoliensinine-treated T24 and UMUC3 UC cells, depicted with the −log10 p -value on the y-axis and the log2 fold change (FC) value on the x-axis. The horizontal dotted line represents p = 0.01, and the vertical dotted lines represent FC = ±0.3. ( C ) GO analysis of DEGs in isoliensinine-treated UC cells, analyzed by DEG counts. ( D ) GO analysis of DEGs in isoliensinine-treated UC cells arranged by gene ratio. Circle size indicates the number of genes involved. ( E ) Hierarchical clustering heatmap of DEGs selected from biological processes in ( C ) in isoliensinine-treated UC cells that cluster DEGs into upregulated and downregulated genes.
Article Snippet: In this study,
Techniques: RNA Sequencing
Journal: Pharmaceuticals
Article Title: Isoliensinine Induces Ferroptosis in Urothelial Carcinoma Cells via the PI3K/AKT/HIF-1α Axis: Molecular Evidence from Next-Generation Sequencing
doi: 10.3390/ph18071008
Figure Lengend Snippet: Isoliensinine affects apoptosis, ferroptosis, the PI3K/AKT pathway, and the HIF-1 signaling pathway in urothelial carcinoma (UC) cells. ( A ) Western blots of apoptosis-related proteins in T24 after treatment with isoliensinine for 2 days and in UMUC3 after treatment with isoliensinine for 4 days. ( B ) Western blots showing the expression of proteins targeting the HIF-1 signaling pathway, the PI3K/AKT pathway, and ferroptosis. Densitometric analyses are presented in in the form of bar graphs.
Article Snippet: In this study,
Techniques: Western Blot, Expressing